In cREMaG, a maximal range of 15 kbp is possible for an analysis starting 10 kbp upstream of the TSS and finishing 5 kbp downstream of the TSS.
Graphing the position of each of these peaks relative to the TSS shows an enrichment in the 200bp just 5' to the TSS (Figure 4).
This suggests that some genes have conserved nucleosomes just downstream of the TSS, and some genes have conserved regions upstream of the TSS.
We find that the distribution of PARP3 is preferentially downstream of the TSS rather than in the promoter region or at the TSS.
Dj stands for the distance between the j-th bin and the TSS, represented through the number of bins away from the TSS bin.
Negative elements resided between -1000 to -500 bp upstream of the TSS, while the positive elements were placed closer to the TSS, between -350 to -40 bp upstream of the TSS in 55% of the genes tested [ 4].
In general, regions and overlap areas close to the TSS are short and increase in length as they go farther from the TSS.
Given the tightly constrained promoter position relative to the TSS (usually between 7 to 10 nucleotides) promoter identification is straightforward once the TSS has been experimentally determined.
The TSS peak height was considered as representative of the level of expression of the TSS.
H3K9ac has a characteristic bimodal distribution around the TSSs, with one single peak upstream of the TSS, a stronger single peak downstream of the TSS, and depletion right on the TSS.
At first she was the TSS Dover, a steam-powered car ferry, but she was soon transformed into a Mediterranean passenger ship called the Sol Express.
