Exact(2)
The triple step is done with three quick steps, starting on your free foot (in this case, your left) in two beats.
The triple step is done with three quick steps, starting on your free foot (in this case, your right) in two beats.
Similar(58)
Then, 30 cycles of the triple steps were performed for 30 sec at 94°C, 30 sec at 52°C, and 2 min at 72°C; the final elongation step was achieved after 10 min at 72°C.
The incubation process of the thermo cycle of the PCR (Geneamp PCR systems 9700; Applied Biosystems, Perkin – Elmer, Foster City, CA, USA) was performed as follows: 5 min at 95°C, 10 cycles of the triple steps that are 30 s at 95°C, 30 s at 60°C, and 30 s at 72°C; then another 10 cycles of the triple steps that are 30 s at 95°C, 30 s at 58°C, and 30 s at 72°C; and finally 5 min at 72°C.
On the third beat, triple step around your lead as he wraps his arm around your back.
For triple step purifications the Strep elutions were incubated for 2 hrs with FLAG M2 monoclonal antibody matrix (Sigma), and then washed 3times with FSH buffer followed by elution with FSH buffer containing 300 µg/ml 3x FLAG peptide (Sigma).
On the seventh and eighth beat, triple step in place.
The director will most likely ask you to perform the Triple Time step, which you can learn by reading the following series of steps.
After the triple wash step the beads were split into three equal aliquots and were incubated for 15 min on ice in RIPA buffer with 150, 650 or 1150 mM total salt, respectively, vortexing once.
While doing this, do a triple step and end up facing the direction opposite of when you started.
"I'll be doing a triple step on every flight," Lunatic said.
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