Exact(7)
Moreover, ~40% of bicistronic transcripts showed negative correlation in the translation levels of their two coding sequences, suggesting a potential regulatory role for these novel regions.
Therefore the two datasets were used to compare the translation levels of TATA and TATA-less genes, containing or lacking uAUG.
UTR start codons can inhibit gene activities at both the transcription and the translation levels (Calvo et al. 2009; Yun et al. 2012).
One argument against using cytoplasmic RNA could be that the translation levels of certain transcripts might be regulated by their transportation rate from nucleus to cytoplasm [ 6, 7].
The authors hypothesized that the augmented production of this protein was achieved either by an increase in the translation levels of the existing mRNA or by a slowing down of the turnover of the existing proteins [ 32].
Our data suggest that miR-26a by itself did not affect the translation levels of 3-UTR'Wee1-luc, but synergistically inhibited its expression when co-transfected with miR-16.
Similar(53)
Finally, we did not detect any evidence for global regulation at the translation level for miR-203 targets based on our analysis of translational efficiency changes upon miR-203 deletion.
By changing the amount of rare codons inserted into ORF1, we sought to change the translation level of ORF1 and subsequently affect mCherry expression in ORF2.
Currently, there are few approaches to precisely detect the expression level of the target protein, not to mention the technique for real-time monitoring of the translation level of the recombinant protein.
The mRNA targets whose levels decreased only modestly may be regulated primarily at the translation level or miRNAs may play only small modulatory roles in the expression of the proteins these mRNAs encode.
On the other hand, based on in vitro analysis of the effect of IRE1α on insulin, suppression of IRE1α has been reported to cause low insulin biosynthesis at the translation level but has little impact on insulin secretion [28].
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