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One of the investigators (NCA) re-coded electronic copies of the transcripts in NVivo 9 (QSR International, Victoria, Australia) using focused, line-by-line analysis based on the "patient-provider relationship" codes identified in the initial round of coding.
With the exceptions of the AS and PRT in D. baltica, we found multiple copies of all the transcripts in the dinotoms (for a total of 73 distinct cDNAs).
Because we can assume that every cell contains approximately one million mRNA molecules, we can consider the values in ppm as copy numbers of the transcripts in a cell [ 27].
Our proposed approach will provide quantitative information on the transcripts at single-copy sensitivity in single cells.
The transcripts of OsNAP in single-copy transgenic lines were detected by Northern blot as described previously [ 2].
The comparison of TE transcripts in the MICROBASE with the genomic copies should be interesting to estimate the impact of the RIP defense mechanism in M. violaceum.
Three segments of the debate Friday between President Bush and Senator John Kerry were omitted from the transcript in some copies yesterday.
Similarly, in nodules of the same INRAT-93 plants, less than 1% of the transcripts were present in very high copy numbers (> 500 copies × 100,000-1).
To calculate the correlation of barcode counts to expected copies of ERCC RNA transcripts in the reaction, we followed the barcode extraction and clustering steps to derive the molecular barcode count for each observed amplicon in the sequencing reads.
It is also notable that the copy numbers of their transcripts in the bacteriocyte were comparable to those of the control transcript encoding ribosomal protein L7 (RpL7), indicating that their expression levels are relatively high.
Lower Columbia College no longer issues paper copies of GED transcripts in the Testing Center.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com