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The transcript consists of 1838 nucleotides, including a 5′-untranslated region (UTR) of 32 bp, an ORF of 1614 bp and a 3′-UTR of 192 bp (GenBank accession number KC176709) (Fig. 1).
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All methods missed the first intron, which was supported by six error-containing reads, but produced partial reconstructions of the transcript, consisting of multiple contigs (Additional file 1: Section S4).
SNPs residing in transcribed RNAs have extensive potential to affect function, because the RNA transcript consists of a single-stranded nucleic acid, which folds onto itself to yield an assembly of structures that determine the RNA's biology.
The short-form transcript consists of three exons and two introns; the middle region of the second exon of the long-form transcript is spliced out as the second intron.
The long-form transcript consists of two exons and a single intron.
The AmphiSyn-long transcript consists of 12 exons (size between 36 and 248 bp) spanning 36 kb of genomic DNA of the scaffold 37.
There was only a small region of high homology with the 5′UTR of the Ensembl transcript consisting of 10 nucleotides (indicated in Figure 1 by ∼) derived from the end of exon 1 (5 nucleotides) and the beginning of exon 2 (5 nucleotides).
One transcript consists of two exons coding for a 1188-residue protein.
The proteins encoded by these transcripts consist of C-terminally truncated DISC1 in some instances fused to 1, 60 or 69 novel amino acids encoded by the DISC1FP1 sequence.
This mutation resulted in the production of an in frame fusion transcript consisting of exons 1 (untranslated region), 2, and 3 from tacstd1, a CD2-derived transmembrane domain, and βgeo.
The active full length NPP7 is a protein translated from 1.4 kb transcript consisting of 5 exons.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com