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In particular, at least 14.78 Mb of indel sequences and 40.75 Mb of unmapped sequences were identified in the Kasalath genome in comparison with the Nipponbare genome suggesting that ~6.3%% of the total transcript loci in rice genome is presumably involved with gain or loss of genes.
Ideally, a complete gene expression analysis would investigate change in both the total transcript level and in the relative abundance of variant isoforms.
Interestingly, transcript levels of katG and srpA in the mid-exponential phase were equal to or even higher than the total transcript levels observed during the stationary or late-stationary phases (Figure 3).
Real-time PCR analysis showed that the total transcript level of both endogenous and transgenic MYB75 in Sense #24 was reduced to only 9% of MYB75 levels in WT.
Differences in the total transcript level (TC) are also given as log2 ratios, as usual.
One competition is for the promoter-specific transcript; the other is for the total transcript.
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In total, these putative B. napus transcription factor genes represent 4.72% of the total transcripts.
Of the total transcripts mapped to glycolysis and one-carbon metabolism, 50%% were up-regulated and 50%% were down-regulated (Fig. 1, Additional file 6: Table S3).
The total transcripts were grouped into 20 clusters of orthologous group categories and summarized in 3 gene ontology categories (biological process, cellular component, and molecular function).
This figure signifies the dominance of biological process followed by metabolic process as GO categories to which the maximum number of transcripts were mapped i.e. more than 80% and 600 %, respectively, of the total transcripts from the microarray data.
Fifty-seven induced (14% of the total) and 13 repressed (4% of the total) transcripts were common to all three fusion proteins (Figure 5 and Table S1).
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