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A new model was developed to estimate endogenous N secretions and losses for the preintestinal, intestinal, and the total sections of the gastrointestinal tract.
Every fifth section was selected from the total sections through the striatum and analyzed to estimate total cell number and striatal volume.
The proportion of tissue area presenting inflammatory cells was estimated over the total sections of the tissues analyzed, using the inflammation scores described by Sun and Tarleton for skeletal muscle: 0 = normal, 1 = scarce cellular infiltrate, 2 = diffuse infiltrate, 3 = abundant infiltrate, and 4 = granulomatous-like infiltrate [ 29].
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In the course of rolling of bar, the total section compression ratio is up to 4.47.
At cell density of 1 × 107 cells/cm3, the 30% c.a of the total section area is under oxygen deficiency (O2 partial pressure below the critical threshold of 38 mmHg).
Tubular injury scores and interstitial cell infiltration scores were accessed based on HE staining and fibrosis scores were assessed based on Masson trichrome staining by using semi-quantitative measurements according to the proportion relative to the total section area and classified as follows: 0 (nil), 1 (<25%), 2 (25–50%), 3 (50 75%), and 4 (>75% of tubulointerstitial fields).
Since it was impossible to discriminate between resident and augmented bone, histomorphometric measurements were performed over the total section of the biopsy, including native and newly formed bone.
The positively stained areas were quantified (ImageJ, Bethesda, MD) and reported to the total section area.
In essence, a squared grid is used to distribute sample points evenly over the total section area, with all sections included in the analysis.
Quantitative assessment of hK1 expression was performed by measuring (1) the section area positive for hK1 in relation to the total section area and (2) the intensity of the staining.
For example, within the measurement section, the methodology for evaluation of exposure (HPV) was allocated 65% of the total section score while the remaining 35% was ascribed to measurement of outcome (OSCC).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com