The total quantification assay was composed of two methylation-unspecific oligonucleotides and a scorpion primer unspecific for DNA methylation.
The reproducibility was determined on the total linear quantification range of both curves confirming the accuracy of the technical set-up in the complete ranges of quantification over time.
Figure 7 summarizes the total of ELP quantification results of the three scenarios constructed in each country.
As the 1.4-kb transcript represented approximately 10% of the total radioactivity, quantification was restricted to the 2.4-kb transcript.
The bacterial growth was determined by quantification of the total proteins after centrifugation of the sample using the Lowry method [ 40].
Analysis of gene family expression included quantification of the total transcript abundance of all isoforms together, as well as the relative contribution of each member.
The added value of the probabilistic assessment is the quantification of total risk from any exposure pattern and the possibility to investigate effects on specified percentiles of species effect combinations.
(B ) Quantification of the total dendritic length of neuronal tracings (mean + SEM, Student's t test), (C ) Sholl analysis of neuronal tracings (mean + SEM, two-way ANOVA and Bonferroni post-test).
TILDA correlated with several PCR-based assays that measure the size of the latent reservoir, including the quantification of total and integrated HIV DNA in PBMCs.
The enzymatic reaction was in the same conditions as for the quantification of total GSH, except that the volume of the derivatized sample was 50 μL.
(E ) Quantification of the total cell number of CD133+ cells in G1 and G7 spheres.
