Sentence examples for the total preparation from inspiring English sources

Second, the total preparation of a pre-game routine includes a good physical warm-up, making sure your equipment is prepared optimally, reaching your ideal intensity, narrowing your focus onto what you need to do to play your best, and grabbing your best mindset.

The total preparation time for each tracer, including purification and formulation, was approximately 30 min from the end of irradiation.

Each of these additional steps add 45-60 minutos thetotalotal preparation time, with about 15-20 min of those being hands on.

The total preparation, baking, and cleanup time should clock-in at about 20 minutes.

A band at 150 kDa corresponding to the β subunit was observed in the whole cell lysate and was absent in the total membrane preparation (Fig. 2D), indicating that the membrane preparation is free of cytoplasmic contamination.

The total sample preparation (reduction and deglycosylation) could be carried out efficiently within two hours.

DNA contamination in the total RNA preparation was eliminated using DNase I (Qiagen).

miRNA was isolated from the total RNA preparation using the Flash Page™ gel system (Ambion, Austin, TX).

RNase-free DNase I (TaKaRa Bio Dalian CO., Ltd) was used to digest the genomic DNA in the total RNA preparation.

Three micrograms of the total RNA preparation were then used for the synthesis of cDNA.

The total RNA preparation isolated was stored at -80°C till further use.