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In 2005 Beagles were involved in less than 0.3% of the total experiments on animals in the UK, but of the 7670 experiments performed on dogs 7406 involved Beagles (96.6%).
The total experiments were classified into three groups, which were miR-125bm (or miR-125bi) group, miR-125bm NC (or miR-125bi NC) group, and untransfected group.
Summary of the total experiments are shown in the 'SUMMARY' sheet in the file named 'Figure 3_data_summaryandexp1_DATA.xls.' All the tif images have 16-bit depth.
For instance, it includes 96 groups of experiments, 78.7% of the total experiments, in Category 3 and Category 4. Furthermore, the x-coordinate is higher than the y-coordinate more than 10% in 17 groups of experiment.
Summary of the total experiments are shown in the 'SUMMARY' sheet in the file named 'Figure 2A_data_summaryandexp1_DATA.xls' or 'Figure 2B_data_summaryandexp1_DATA.xls.' The same set of data for experiments with the wild-type strain are shown graphically in Figure 2 figure supplement 3 in the supplemental materials.
In particular, the left Pie Chart is divided into 4 parts corresponding to the percentage of each category and Category 1 is not described in the Pie Chart due to its percentage of 0. For instance, Category 5 in Table 5 indicates that there are 30 experiments with the agreement between 0.8 and 1, which accounts for 24.6% of the total experiments.
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CL carried out the total experiment and wrote the manuscript.
Finally, contaminated bacteria or DNA may fail the total experiment.
The total experiment took about 1 h to complete.
The total experiment lasted typically 31/2 h, depending on quality of the output signal and the stabilization period.
By eliminating both the pre-test and post-test, we can further reduce the total experiment time.
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