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The tight adherence to Gin's present tense consciousness slides into a past tense voice, pushing readers away from the story's dénouement.
This was also confirmed by the user questionnaire and by the tight adherence of healthcare professionals to the suggested insulin doses.
We demonstrated that, due to the porous structure of the 3D scaffolds and the full infiltration of nutrients, the PGA treatment facilitated the tight adherence of pancreatic islets to the scaffold fibers and had the prominent effect of reversing the decrease in cell viability observed in the static culture condition (P < 0.05, Figure 2).
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The BS001 genome also revealed the presence of two copies of a subtype of the T2SS, the Tad (tight adherence) macromolecular transport system.
The very tight adherence to nucleated cells is mediated by the interaction between YadA and β1 integrin receptors but occurs indirectly through an integrin-extracellular matrix protein YadA linkage [29], [30], [31] (for review [32]).
The unique genes include potential antibiotic resistance genes, CRISPR-related proteins, and members of the Tad (tight adherence gene) macromolecular transport system that may indicate that the secretion systems used by DSM 24701 are different (Additional file 5: Figure S4).
This allows for: preservation of the integrity of the posterior tracheal wall, no traumatism of the peritracheal tissues, tight adherence of the stoma to the tracheostomic canula, irrelevant bleeding.
The tad (tight adherence) locus is widespread in bacteria and is involved in adhesion or secretion [ 25].
Unlike for Tfpb that are more heterogeneous Flp that are assembled by the conserved tight adherence (Tad) locus are an exception (Tomich, Planet and Figurski 2007)—genes are conserved 'en bloc' for Tfpa or T2SS with similar genetic organizations.
The CDSs of the Flp-type pilus are harboured on GI-VSA5 and show sequence similarities and high synteny to the Tad (tight adherence) macromolecular transport system of Actinobacillus actinomycetemcomitans.
All but two of the promoters show very tight adherence to the WWATAGGTA consensus (Fig. 2c); the TU1 promoter upstream of the RNL gene is the only one, where the +1 nucleotide is G instead of A, whereas the putative transcription start site of TU7, upstream of NAD4L significantly deviates from the consensus at several positions, yet has strong experimental support in RNA-seq and 5'-RACE results.
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