Exact(11)
We isolated the three nuclei of a mature pollen grain manually.
In this case the three nuclei could be released immediately from the pollen by osmotic pressure, unlike with CAU5.
In this study, we isolated the four microspores of multiple tetrads and the three nuclei of mature pollen grains of the CAU5 line for whole-genome sequencing.
In the present study, we make further efforts to improve the method for isolating the three nuclei from one mature pollen grain of maize for whole-genome sequencing.
The three nuclei of each pollen grain were placed in individual tubes and lysed for whole-genome amplification, then the genome was sequenced.
After 15 min, the three nuclei could be observed in a pollen grain with SYTOX Green fluorescence under × 10 microscope (Fig. 2a, b).
Similar(49)
…with the two nuclei (polar nuclei) within the large central cell of the female gametophyte.
So that is the bond axis -- it's just the axis between the two nuclei.
The four nuclei and their derivatives undergo repeated mitoses without cell-wall formation.
The two nuclei each divide, and then one of the nuclei from each end of the sac moves to the middle.
This process, if accompanied by homologous recombination as in meiosis, could be essential for maintaining homozygosity of the two nuclei.
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