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The third swab was inoculated immediately into the culture tube containing modified Diamond's medium.
These colonies could not be demonstrated in the culture of the third swab from the outside of the erythema.
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The remaining 135 tadpoles reached Gosner stage 42 (beginning of metamorphosis) 25 to 129 days after 25 April, when the first swab was collected (and 32 136 days after capture).
For group 2 (n = 133), the first swab was placed in transport medium and the second swab was placed in the empty vial (dry swab).
The first swab was placed in a vial containing AMPLICOR Specimen Transport Medium of Roche and the second swab was placed in 1 ml transport medium.
(The mean time between the first swab and the home visit was 2.5 weeks).
The second swab was placed in a dry cryotube and stored at −80°C for future analysis.
The first swab was rolled onto a slide for Gram staining; the second vaginal swab was used for bacterial genomic DNA extraction.
The second swab (Affirm hardwood swab; Becton Dickinson, Heidelberg, Germany) was used for DNA hybridisation and PCR analysis.
If the first swab was not tolerated a second swab was performed or nasal secretions were collected from a tissue used to blow the nose [ 15].
Following the incubation period, the second swab was processed as described for the first and the results were compared.
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