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On the third passage, UCMSCs were induced to differentiate into dermal fibroblasts in conditioned induction media.
The flow finally redirected to radial outward by the 180° hub turn and discharges through the slot configuration at the third passage.
FACS analysis of hADSCs at the third passage showed that these cultured cells were positive for CD13 (98.88%), CD44 (98.9%), CD59 (98.4%), and CD105 (71.24%).
Cells were used in experiments after the third passage.
After the third passage, cells have been used for subsequent in vitro and in vivo experiments.
Confluent cells (approximately 2×106) at the third passage were used for the experiments.
Optical microscopic analysis up to the third passage showed that this differentiated morphology did not alter on subculture.
Virus recovered from the third passage exhibited the highest level of HA expression and was subsequently used for further studies.
At confluence and after the third passage, cells were also immunolabelled against SMCs and ECs specific markers.
Cells at the third passage were used for experiments.
The third passage of ADSCs was adopted for immunophenotype analysis.
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