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One mouse was injected 90 days after the third injection with 60 µg EBNA1 contained in PBS, administered IP.
In the third injection (left hemisphere of BRh3), FB was injected into the mid and caudal anterior medial nucleus (Fig. 9).
All the cycles except the third injection required only ambient or very moderate pressures.
With the third injection, the dye flowed into the disk and started dribbling out through a tear.
One week after the third injection, the mice were euthanized, and single-cell suspensions of splenocytes were prepared.
After the background correction, the curve relative to the second injection was fitted and its contribution subtracted to the third injection.
Blood samples were taken at 2, 4 and 24 hours post dosing; at 2 hours post the second injection, and at 4 hours post the third injection.
In went the third injection and a tear trickled out.
The volunteer received the third injection following his recovery.
The rabbits were bled six weeks after the third injection for preparation of antiserum.
After the third injection of the fusion proteins into Swiss Webster mice, ascites fluid was collected and used for immunostaining.
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