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One week after the third immunization, serum antibody titers of immunized mice were determined by indirect ELISA on inactivated E. tarda or OMP-coated plates.
Antisera were used for following experiments after the third immunization.
b One week after the third immunization with OVA and alum, mice were euthanized and single-cell suspensions of splenocytes were prepared.
The frequency of SIV Gag- and SIV Tat-tetramer positive CD8 CTL in the intestinal mucosa reached 30 50% after the third immunization.
As shown in Figure 2A, the titers of the antisera (indicated by the absorbance values) increased with the repetitive immunizations, however, rabbit No. 1 showed weak response and rabbit No. 4 has been lost after the third immunization.
Moreover, when ECG traces were obtained from immunized mice, the heart functional alterations observed at 10 days continued at 80 and 150 days after the third immunization, showing an association with the levels of antibodies.
Nevertheless the third immunization was withheld.
Preimmune serum samples or serum samples after the third immunization of individual mice were pooled for each group.
Blood was collected from all groups four weeks after the third immunization dose and just before intranasal challenge.
Of these five volunteers, three reported systemic events after the second immunization and two after the third immunization.
There was a demonstrable increase in IFN-γ production after the third immunization in all vaccine groups.
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