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Herein, GOxNPs, hemin/G-quadruplex and PtNPs could form mimicking bi-enzyme cascade catalysis system to in situ generate dissolved O2 as coreactant in peroxydisulfate solution when the testing buffer contained proper amounts of glucose.
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The test buffer (110 μL/well) was applied onto the micro titer plates and the enzyme lysates (40 μL) were added.
As the sensitivity of the blood assay might be improved in the same way, this possibility has been examined under both laboratory and field conditions, by adding EDTA to the test buffer or, as an anticoagulant, to the blood samples.
Briefly, 15 μL of undiluted serum sample is added to the sample well, and 220 μL of testing buffer to the buffer well.
Blank control well was added with 10 μL homogenization buffer, 90 μL testing buffer, and 100 μL testing reaction solution.
The 10 μL muscle extract was added into a 96-well flat-bottomed plate; then 90 μL testing buffer and 100 μL testing reaction solutions were added into each well.
The subsequent reduction with NaBH4 and quantification of the formed 5,6-dihydro-M1dG showed no difference among the three tested buffers, suggesting no effect of Tris-HCl on the sensitivity of our assay.
The further tested buffered formulations included two reference batches from the BE studies (Lipitor and Sortis10), plus one other batch of the reference product, Sortis06.
The acidic and alkaline partner solutions (each having 10 mM lithium ion) were mixed at room temperature to achieve the desired pH of each test buffer.
In order to quickly test buffer systems if the evaporation of electrophoresis solutions is acceptable, an alternative experimental design without doing CE experiments had to be found.
Fifteen filler pictures were also placed at the beginning and end of the testing pictures as buffers and two of these were also presented upside down.
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