Toluene was used as the test compound.
A schematic summary of the test compound selection is described in Supple.
The procedure involves using a range of concentrations of the test compound in one 96-well microtitre plate.
n-Butane was employed as the test compound for obtaining the experimental breakthrough curves.
The test compound or control substances (succinate, dimethyl succinate, monomethyl succinate, malonate, dimethyl malonate or dimethylsulphoxide (DMSO)) were dosed as indicated in each figure.
The test compound was added 24 h later in the presence of calcium, and viability was determined after 4.5 h of incubation.
Additionally, where indicated, increasing doses of Auranofin (AFN, #BML-EI206-0100, Enzor or Aurothioglucose (ATG, #A0606, Sigma Aldrich) were co-incubated with BSO and the test compound.
For compound screening and target identification measurements cells were treated with 200 µM BSO alone or in co-incubation with semi-logarithmic dilutions of the test compound.
In addition to the test compound in row 1, other wells contained GAST/Fe medium, 5% DMSO, rifampicin and kanamycin as controls.
The cells were then treated for 72 h (or shorter as indicated) with culture medium containing semi-logarithmic dilutions of the test compound.
In oviposition deterrent experiments against Ceratitis capitata, we observed a moderate effect at 30 μg/cm2 of the test compound.
