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We have successfully extended the identified N-terminal annotation of the 486 proteins detected in R. pomeroyi to 9887 homologous genes in the thirty-six sequenced Roseobacter isolates, corresponding to nineteen distinct genera.
By focusing on the terminal nodes, the annotations provided a clearer picture of the potential biological functions governed by G-proteins and their interactors.
5' and 3' RACE experiments were needed for correcting N- and C-terminal annotation errors.
The closer the nodes are to the terminal, the more detailed annotations are given and thereby are more informative.
It can be difficult to build primers which lead to sufficient duplication of the terminal regions of genes, thus effective annotation of these exons by computational means can save researchers invaluable resources.
Most of the differences were due to differences in N-terminal annotations, but 5 of these 24 proteins were also specific to Sanger, while their peptides and the corresponding genes were not annotated in the TIGR database.
The terminal was nearly empty.
The terminal is clean, though.
The terminal later reopened.
Back to the terminal once more.
The terminal degree must be recent.
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