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top Detection of RNA transcripts in vivo from the templates shown in Figure 2A by LNA probe-hybridization.
However, we observed barely any improvement yielded by the additional constraints, as shown in Figure 3 obtained from Supplementary Tables 4 and 5. Of the templates shown in blue italic in Supplementary Tables 4 and 5, 32 have outer atoms, and therefore might give different predictions.
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It has been found that the group using the template shown average reduction in time of about 3.39 min. This time reduction is very much of importance at the time of examination.
A maximum carbon concentration may probably occur over the around center of the nanopores, as the schematic simulated alternative carbon concentration curve over the template shown in Figure 6b.
To do this, we used LNA probe-hybridization to detect transcripts produced from the template shown in Figure 1B.
The alignment in Figure 2a shows that the motif RNDNR107 in R277 (shown in light pink) is not fully aligned and is six residues away from the motif RHNNR461 of the template (shown in dark pink).
This λPR′ template bears the same −10-like element as the template shown in Figure 1A, but in addition carries a promoter-proximal −10-like element (positioned between +1 and +6) that induces σ-dependent pausing at a nascent RNA length of ∼16 nt.
To do this: Use the template shown in this article.
Even if you don't have a printer, you can easily copy the template shown here by free hand and cut it out to follow as a template.
It may help to draw the lines indicated by the arrows first, then cut (print out the template shown here so that it's easier to follow).
Sequence alignment with several FIC motif containing proteins, complemented with homology modeling on the FIC motif containing protein, VbhT of Bartonella schoenbuchensis as the template, showed conservation and interaction of residues constituting the FIC domain.
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