The first step introduced was a real-time PCR, which amplified a genomic region containing the SNP in order to develop the template needed for the ensuing LCR protocols.
To count as performed, the template needed to either have the relevant item checked/circled/slashed (if it was a pre-printed item) or have the item and results of the assessment handwritten on to the template.
Although this alleviated some of the challenges, the template needed further refinement to incorporate information on other sources of funding available to the applicant, at what stage the study is, institutional capacity, and budget justification.
For microarray analysis, RNA aliquots of 4 controls and 4 affected samples were pooled to reduce the template needed and to exclude differences in expression due to individual variation and not related to BSP [ 5].
Remember that not all articles with the template need to be deleted.
Of course, when building homology models using the combinations of TMHs shown in Table 7, the templates need to be superimposed first (e.g. using the seven highly conserved residues as reference points).
In order to achieve this objective, the hollow template needs to be applied.
This is not to say that the India experience provides the perfect template needed to address the situation in Pakistan.
In most cases the semantics of a given data source are not (yet) described in machine readable form and the initial mapping template needs to be generated manually.
