Exact(1)
Co-IP showed that the deletion mutants of SLX4 (SLX4ΔMBR, SLX4ΔMBR, and SLX4ΔSBR), each lacking only the specific interacting region for one endonuclease, specifically abolished only the targeted interaction in cells.
Similar(59)
The generation of high-fidelity engineered tissue constructs is based on the targeted interactions of organ-specific cells and intelligent biomimetic scaffolds which emulate the natural environment of their native extracellular matrix, in which the cells develop, differentiate and function.
In this study and in general, different bioassays generally means different targets, where "target" can refer to protein target, or a targeted interaction in a phenotypic screen.
The current approach reported in this paper is suitable for enrichment and detection of unknown proteins because the preferential enrichment and detection is governed by the surface properties of the NPs as opposed to abundance sensitive detection in MS and the targeted antigen antibody interaction in the case of WB/IP.
Implementation was approved as a protected web-based platform to allow for targeted interaction, with a first year aim of evaluating this.
We note that the transport paradox goes far beyond the relevance for the transport mechanism, since transient, but targeted interactions are central to the emerging view of highly dynamic protein (and other biomolecular) interaction networks.
We analyzed read-through from randomly integrated expression cassettes because we could not predict how any particular configuration of the targeted integration site would affect the interaction.
More generally, we noted that peptides designed to specifically disrupt an interaction disrupted only the targeted and expected interactions in Astro#40 and U87 cells (2: Figure S2).
If the goal is solely to correct inaccuracies in interview transcripts then a modified process involving researcher verification of the interview transcript, as was also done in this study, followed by targeted interactions with interviewees to clarify any outstanding errors, inaccuracies or omissions, is likely a more appropriate approach than ITR.
For the targeted two-QTL interaction mapping, the markers with a p-value < 1.0 from single environment QTL mapping and the scale and crossover markers obtained from both GEI and slope QTL mapping at a p-value < 1.0 and a distance less than 60 kb were collated.
Conversely, the targeted interference with interactions of proteins or the interactions of proteins with DNA, important, for example, for the survival and proliferation of tumor cells, can be used for drug discovery and development.
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