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The material quality increased with the target length in helium.
On the contrary, poor quality product was obtained whatever the target length in argon.
The target length is 1.5 m with a radius = 5 cm and is facially irradiated by the beam.
The structure of the products was studied by SEM, TEM and Raman spectroscopy as a function of the buffer gas (helium or argon), the target length and the collected location.
Although not one of the longest loci used for STR genotyping, it was chosen as a good compromise given the target length limitations on qPCR efficiency with TaqMan® detection.
To test the impact of the target length on MIP performance, before designing MIPs we randomly deleted codons from 65 contigs; actual targets for MIPs designed in these contigs were longer than the standard 112 bp, varying from 115 to 154 bp and in one case 235 bp.
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The target lengths L, heights H and speeds V are chosen as the most typical for each particular target.
Bands of the target lengths were excised from the gel, and DNA was extracted using the QIAquick Gel Extraction Kit (QIAGEN, Netherlands).
From such results, the determination of the characteristics of the targets (length and width) is not trivial.
For example, during the first 400 ms of this particular trial (the 10 frames before start of the first oscillation), the target-length standard deviation is 0.4%.
Since the LSF is the integral of the PSF along the imaged slit the reliable angle from which the stray light could be determined is governed by the object angle of the slit target length and collimator focal length.
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