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Primer concentrations and annealing temperatures were optimized for the iCycler iQ system (Bio-Rad) for each set of primers using the system's gradient protocol.
Calibration of bone mineral density (BMD) was carried out according to the system manufacturer's protocol.
The court decided that the commission was right to fine Microsoft euro497m ($690m) for "bundling" its media player with Windows and for refusing to share data with rivals so they could comply with the operating system's protocols.
Early last year, they began working under the system's disease management guidelines, or protocols.
With Republicans in Congress pressing for a further expansion of the system, the protocol's lack of specificity may well stoke the Russian fears of facing a slippery slope to a larger system.
Total protein was isolated from whole microdissected HFs using a specific buffer from the Human Phospho-Kinase ARY003BAR&D03B, R&D System), following the manufacturer's protocol.
Quantitative real-time PCR (RT-qPCR) was performed with mammalian Taqman probes (β-actin, ACTB [Hs01046520_m1]; MYC [Hs00905030_m1]) (Applied Biosystems) using the Applied Biosystems 7500 Real-Time PCR system following the manufacturer's protocol.
Proteins were visualized using an ECL chemiluminescence detection system, following the manufacturer's protocol and Hyperfilm ECL (Amersham).
Quantitative RT-PCR was performed using the ABI 7500 real time PCR system following the manufacturer's protocol.
RT-PCR analysis of selected genes was used the SuperScript One-Step RT-PCR System following the manufacture's protocol.
Finally, mRNA libraries were sequenced on the Illumina Genome Analyzer II (Illumina Inc, US) system following the manufacturer's protocol.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com