On the other hand, Notch1 receptors lacking the S1 loop exhibit a substantial defect in transport to the cell surface, with steady state cell-surface levels reduced by 5 10-fold, even though there are comparable levels of overall expression.
MHs are considered complicated by the authors when one of the following apply: (a) a history of MH for 6 months or more; (b) after primary or even secondary retinal surgery, for example, when short-term endotamponades, such as SF6 or C3F8, were unsuccessful; or (c) whenever a larger central substantial defect was appreciated when the indication for the operation was established.
However, various features of the disorganized cortex indicated a more substantial defect of cortical layering (Caviness and Rakic 1978; Rakic and Caviness 1995).
We also monitored possible effects of A2ML MO injection on blood vessel formation in the liver by inspecting fliGFP transgenic animals, and found no substantial defect in blood vessel formation (Fig. S2A B).
However, in the case of the DWCNTs fluorinated at 400 °C, the recovery of strong RBMs from the inner tube and weakened RBMs from the outer tube indicated the selective introduction of substantial defects on the outer tube while preserving the original tubular shape.
We next monitored the accumulation of RAD51 foci following irradiation and found a more substantial defect in INO80-depleted cells (Fig 2B).
The C. neoformans met3 mutant, which lacks ATP sulphurylase activity, had a substantial defect in melanin formation, significantly reducedgrowth rate, and greatly increased thermotolerance.
Surprisingly, our own more detailed analyses did not reveal any substantial defect of osteoclast-specific gene expression in PLCγ2−/− cultures, and the expression of NFATc1 was not at all affected by the PLCγ2 mutation (Fig. 4b).
The sspA-S47D ∆ sspB strain was perturbed in sporulation, producing spores with a substantial defect in UV resistance in comparison to ∆ sspB spores (Fig. 3d; Additional file 10: Table S6).
The spt16-m allele contains two amino acids substitution (K692AR693A) in its tandem pleckstrin homology (PH) domain and displays a subtle defect in transcription initiation but a substantial defect in DNA replication-coupled nucleosome assembly (Yang et al., 2016).
