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The stringency is also discussed in the article.
The stringency is the percentage of matches and mismatches between a probe and target nucleic acids that are allowed to occur without the double helix hybrid falling apart.
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Then, the stringency was decreased from 1e−45 to 1e−15 with the 'Ends to Ends' auto merge function.
Finally, the 'keyset to functionction was used and the stringency was adjusted to 1e−25 with a minimum of 2 ends matching.
The stringency was set at ≥4 fold with the intent of identifying the smallest number of miRNAs with the highest probability of contributing to VERO cell neoplastic development.
As shown in Figure 4, it was hard to differentiate CD24 heterozygotes from homozygotes at low stringency, but separation gradually improved as the stringency was raised, as would be expected for a probe pair that is functioning correctly.
The stringency was increased thereafter to limit the number of unique reads to four.
Then, the stringency was decreased at nine successively lower cutoff from 1e-60 to 1e-15.
For loci used for either map, the stringency was stepped down and the map locations were compared.
Next, the "Ends to Ends" auto merge function was used with default settings and the stringency was decreased from 1e-40 to 1e-15.
Signal loss was initially evaluated at 50%% and the stringency was decreased in increments of 10%% until reaching a 20%% loss of signal.
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