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The strain was maintained on solid medium at 4 °C.
The strain was maintained on Sabouraud's dextrose agar (SDA).
The strain was maintained as spore suspension in 5% corn meal medium at 4°C.
The strain was maintained frozen at −80 °C with 20% (w/w) glycerol added.
The strain was maintained in the Food Biotechnology Laboratory at Ningbo University.
The strain was maintained at − 80 °C in 20% sterile glycerol until needed.
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On the contrary, if the strain is maintained fixed during unloading, the specimen returns instantaneously to the ambient temperature.
Indeed, if the strain is maintained fixed during loading, the temperature continues to increase for a few seconds before returning to the ambient temperature.
The strain is maintained on VERO Cells (African Green Monkey cell line obtained from ATCC on 2006), grown in monolayers in RPM-I1640 medium, supplemented with 10%% heat inactivated FBS.
The strain is maintained through heterozygous matings since females are poor breeders and males are infertile.
E. Dougherty took its descendants to California, where two stocks of the strain were maintained from 1956 by serial transfer, one in axenic liquid culture and the other on nutrient agar inoculated with E. coli [17].
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