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The strain performance and downstream process greatly impact the economics of biobutanol production.
Finally, we suggest further works required to improve the strain performance suitable for successful commercialization of fermentative succinic acid production.
The key points arising from the literature review are illustrated with empirical examples of the strain performance and durability of one of the most popular DEs, VHB 4910.
In summary, the deletion of APJ1 in GSE16 was not detrimental for the strain performance when fermenting spruce hydrolysate.
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In this work, the strain E. coli LA02 was engineered for isobutanol production by combining NADP+ dependent Ehrlich pathway and biosynthetic KIV precursor pathway, but, as the key limitation for isobutanol production, the imbalance redox status with excess NADH and less NADPH supply should be solved to improve the strain performances.
As a control, strains ISA1000 and S288C were grown in the same medium: the inclusion of additional nitrogen sources did not disturbed the strains performance.
During our design of both the succinate and the BDO producing strains, we evaluated the strains' performances in batch reactors.
While the majority of these differences may be attributable to the translation of a truncated CRE1 protein in strain RUT C30, the gene expression kinetic patterns observed suggest that other mutations may also have an effect on the strains' performances and/or transcriptomes.
The FEA simulation, set up under accurate boundary conditions, is carried out to investigate a variety of proposed design structures so as to obtain a scientific understanding on the SAW strain performance of the sensors when being applied as the cutting force sensors.
Additionally, in applications where the engineering goal is to maximize profitability, financial metrics can replace the consolidated strain performance as the strain selection criteria in Steps 4 and 9 of the DySScO algorithm (see Method).
Although richer nutrients and more initial cells improved the fermentation of the hydrolysate, strain performance was still a distinct disadvantage.
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