Here, the main purpose of the analysis was to introduce all identified mutations in strain F into the genome of the parent strain, instead of constructing mutant strains with all possible combinations of mutations.
We thank Dr. Y. Y. Gleba (Icon Genetics GmbH) for generously providing the strain of A. tumefaciens with GFPi construct.
Recent global transcription machinery engineering (gTME) demonstrates the feasibility of constructing strains with superior fermentative capacity (Alper et al. 2006).
Finally, instructors can use the Polley and Fay article to illustrate the utility of model organisms, the ease with which they can be genetically modified, the ease of constructing strains with multiple mutant alleles, and the ability to knock-down gene expression experimentally.
In the process of constructing congenic strains, the QTL allele from the OXYS strain was introgressed into the WAG strain, which showed no signs of cataract and retinopathy development.
The strain was constructed by deletion of the succinate dehydrogenase (SDH) subunit, which induces accumulation of succinic acid in Y. lipolytica, and also in fermentative yeasts as S. cerevisiae and Kluyveromyces lactis[ 28, 63, 64].
Consequently, the strains of two optical extensometers constructed on these two surfaces are obtained with common 2D-DIC, and averaging these strains can eliminate the effect of bending moment and out-of-plane motion of the specimen.
Luria-Bertani broth (L-broth) was utilized for the cultivation of bacteria at 30°C and 43°C when constructing the strains of JM83-AAT24, JM83-ATT24 by using pKOV integration [44], [47]; while all other types of cultivations were carried out at 37°C.
The strain FYJ246 was constructed by P1vir transduction of strain FYJ241 using a lysate grown on strain MFH8 (ΔfadR::Tn10) with selection for tetracycline resistance.
To cure the strains of the replicating plasmid constructs, the strains were passaged twice a day in 20 ml of BHI broth for five days.
