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The starting volume of the reservoir solution was 1 mL, and the volume of the drop was 20 μL of protein and reservoir solution in a 1 1 ratio.
The second aqueous phase was discarded and the detergent phase was resuspended in a volume of buffer, without detergent, equal to the starting volume of lysate used for fractionation.
The starting volume of BAL fluid was in the range of 2 to 5 ml.
The DNA was precipitated again [ 41] and resuspended in TE to the starting volume of the DNA amplification reaction.
The volume of whole blood and the blood fractions derived from the starting volume of donor blood used for transfusion are shown in Table 1.
In contrast, evaporation of two-thirds of the starting volume of liquid CeMM in 75 cm2 flasks is observed to take four to five weeks.
Similar(47)
The starting volumes of urine sample aliquots ranged from 20 to 50 mL.
STX140 (20 mg kg−1 p.o.; daily) caused significant regression (P<0.05) after 28 days relative to the starting volumes of the tumours.
We applied SNV stocks to a 100-kDa cut-off Amicon centrifugation filters and collected the filtrate and retentate, both of which we resuspended to the starting volume by the addition of cell culture medium.
After 21 days of growth, the cultures were filtered and concentrated using a freeze drier until a reduction to 1/10 of the starting volume was achieved.
The pelleted cell debris were dissolved in 1/100 of the starting volume in PBS and stored in −80°C until used.
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