Suggestions(1)
Exact(7)
To determine which isoforms of Dync1i2 were expressed in these tissues, the RT-PCR products were subcloned and sequenced; subsequent analysis, revealed a total of 11 different isoforms each having either exon 1a or exon 1b at the start of the transcript (Table 1, Figure 4).
(3) If there is no matched tissue distribution, repeat the search in the 2 kb sequence downstream to the start of the transcript.
Sequenced PCR products, cloned after amplification of the start of the transcript, included 0, 206 or 370 nucleotides upstream of the predicted translation initiation codon.
Boundaries for 573 CDSs were extended or shortened based on SL mapping of the start of the transcript by using the 5′-most in-frame start codon after the major SL site unless ribosome profiling or mRNA levels indicated otherwise.
However, the Streblomastix intron is located very close to the start of the transcript, so we cannot exclude the possibility that this intron sequence is incomplete and a canonical boundary lies upstream.
Both Gambierdiscus transcriptomes examined here also contained numerous other putative polyketide domains (Fig. 1, Additional file 1: Tables S8 S9), including a transcript resembling a type II KR domain (Fig. 3b, a SL at the start of the transcript confirms its dinoflagellate origin).
Similar(53)
Given that the elongation rate is not homogeneous along a gene, and that promoter-proximal pausing of RNA polymerase II is common, we reasoned that the elongation rate of the polymerase may be generally lower at the start of the transcripts, allowing better recognition of cassette exons that are close to the TSS.
There were 22 genes for which annotation of alternative promoters was based solely on nested transcripts, where the start of the shorter transcripts lay within exons of the longest transcript, e.g. fkh-9.
The Berkeley Gene Disruption Project fly line Sod3 KG06029 has a KG insert just before the start of the SOD3 transcript.
Our results suggests that, using the GenomeTraFac web tool, we can identify several candidate transcription factors that may participate in tissue-specific expression of miRNAs by the following workflow: (1) Start from the 2 kb sequence upstream to the start of the miRNA transcript, and start from the regions with peaks of "Hits" larger than 30.
CpG island 46 on the PITX2 BAC is not annotated as the start of the PITX2 transcript, so we analyzed the DNA methylation levels at eight additional locations on the BAC including exon 1 and intron 2 (non-CpG island), three internal CpG islands, the promoter CpG island of the annotated alternative isoform, and two intergenic CpG islands.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com