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In the procedure proposed herein the standardised sample removal from an in-service component for SP tests is exploited as external action altering the residual stress state possibly present near to the surface in the location considered.
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The concentration of testosterone, and potentially also AMH, varies throughout the day, but due to the standardised sampling, this should not affect the results.
This might be due to differences in the histopathological subtypes of the study subjects, or the lack of standardised sample collection and storage methods.
Complications also arise from the lack of standardised sample collection and isolation methods, which have a dramatic impact on the results.
As many parameters may affect S1P release into the samples after the actual blood collection, standardised sample handling procedures need to be used to obtain reproducible results when comparing both inter-study variation as well as variations between samples collected from different patient groups.
To compare the results of two different TaqMan plates with each other, the same standardised samples are analysed on every plate.
Serum samples were collected using a standardised sample collection protocol and stored at −80 °C until use.
In conclusion, we have developed a highly sensitive and specific LC-MS/MS method for measuring S1P in biological samples, providing in addition a standardised sample collection and preparation protocol.
In this regard, the integrated deposition measured by the standardised PMF sampler reflects an average leaf deposition rate over the flowering period.
The resulting uncertainty factor of 0.0376 was applied in addition to the DC factor to reduce the measured deposition data from the standardised technical sampling to 0.94% (0.25 × 0.0376 = 0.0094 = 0.94%) of initial values.
The main strengths of the GECOH study are the standardised blood sampling conditions, the comparison of different laboratory methods with the parallel evaluation of the AARR and ARR in diagnosing PA and the fact that all patients are scheduled to undergo the screening and the reference standard test.
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