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Both, VRK2A and VRK2B, were able to form a stable complex with JIP1 (Fig. 4A), and the kinase activity was not necessary for the stable interaction since kinase-dead (K169E) proteins also interacted although in a stronger manner.
We could confirm EMC1 GFP interacts with dPob, but we failed to show the stable interaction between EMC1 GFP and Rh1 apoprotein accumulated in the ER in the VA-condition.
The electrochemical stability of Ba-substituted Li10GeP2S12 can be attributed to the structural stability resulting from the stable interaction between Ba2 + and S2 − in the structure.
These results indicate that the stable interaction of PB2 with α-importins is host-dependent.
But the stable interaction was observed in the apo form between TM5 and TM10, whereas in glucose bound form, it was seen between TM5 and TM11.
To evaluate the effect of the stable interaction with β-arrestins, we truncated WT and mutant receptors after serine 331 (331Δ).
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The stable interactions established by LRP-1-ICD with ERK and JNK may influence the ability of MAPK to reach their downstream targets and may have a repercussion for spatial and temporal propagation of intracellular signaling.
Given the stable interactions that we observed for TRIAD1 and HHARI with neddylated cullins, we asked whether TRIAD1 and HHARI might affect CRL neddylation in cells.
Disruption of TcR/pMHCII blocked the stable interactions between T cells and DCs (Videos 1, 2, Figure 7A,B) and increased T cell motility (data not shown).
A second, quantitative system based on fluorogenic galactosidase activity confirmed the ZmHP2 stable interaction, and indicated, on the basis of the quantification of galactosidase activity, that the interaction of ZmHP2 with ZmTCRR-1 is more stable than with ZmTCRR-2.
The resulting stable interaction between a T cell and its cognate APC is the formation of a synapse between the two cells, generally referred to as the immunological synapse (IS) [ 2] owing to its similarity to the neuronal synapse [ 3].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com