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Residues important for the stabilization of the complex were identified using Baker's alanine scanning procedure [31], [32] and the Robetta web server (http://www.robetta.org).org
The modeling predicts that the N-terminus of Ets-1 makes important contributions to the stabilization of the complex, but remains largely disordered.
LUBAC drives linear polyubiquitylation of several components of the TNF-R1 complex [19], which promotes the stabilization of the complex [20] and is essential for the recruitment of NEMO and activation of NF-κB [21].
However, it retains a substantial affinity for HP that might result from the stabilization of the complex through the collaboration between the conserved BBXB motif in the core of the chemokine and the remaining positive charges in the yet highly cationic C-ter domain.
The stabilization of the complex in presenescent VSMCs suggested that it may have a role in DNA damage signalling.
These include how the enzymatic activity is affected by modifications of the H3 tail, as well as the stabilization of the complex by AEBP1.
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This may be due to the stabilization of the complexes due to the hydrophobic interactions between the LA moieties.
Second, the stabilization of the HDAC4 MEF2D complex indirectly stabilizes the association of HDAC3 with MEF2 (Fig 2D lanes 3 and 9), thereby providing an enzymatically active HDAC (Fig 3A).
However, while the DNA strand incorporation decreases the stability, the 2′-F modified RNA increases the stabilization of the 3WJ complex, as shown by the lower dissociation constant compared to the 3WJRNA.
Subsequent interaction with the ligand induces flap closure and the stabilization of the macromolecular complex.
It was also established that the use of methanol as cosolvent significantly improved the assay sensitivity through the stabilization of the ternary complex.
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