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Scheme of the SSPE procedure used in the present study (with DCM, dichlormethane; EA, ethyl acetate; MeOH, methanol; FA, formic acid; NH3, ammonia).
Due to the sequential arrangement of Oasis HLB and C18ec in the SSPE design, there is a significant overlap in the extraction spectra of fractions 1 and 2 because Oasis HLB and Chromabond C18ec are partially able to extract the same classes of organic xenobiotics [23, 24].
Under the assumption of a broad and most complete extraction of the organic wastewater pollutants by the SSPE and fractionation protocol used in this study, total toxicity caused by organic xenobiotics in wastewater could approximately be ascertained by testing the recombinant fractions (Figure 5).
The SSPE proposal was approved by the CPSP Steering Committee and funded by Health Canada.
Through the Canadian Paediatric Surveillance Program (CPSP), the SSPE study was able to document a national incidence and determine the epidemiology of affected Canadian children.
The V357I mutation was one of the fixed changes that we had detected in the 1998 outbreak, but one of the SSPE cases (SSPE5) had a nonconservative V357S change at the same position (from hydrophobic to polar without charge) (9 ).
Similar(54)
Phylogenetic relationships clustered the three SSPE strains with the wild-type D6 genotype.
Arrays were then washed according to the Agilent SSPE wash protocol, and scanned on an Agilent scanner.
On the other hand, this SSPE procedure ensures that there are no gaps in the extraction spectrum which may occur by a parallel arrangement of the SPE sorbents.
The decline in SSPE diagnoses builds on the growing evidence of the success of vaccination in decreasing illness and death from measles and its complications (26, 27 ).
The decrease in SSPE, a neurodegenerative disorder caused by the persistence of measles virus in the central nervous system (25 ), reflects the success of immunization in Australia.
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