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All samples were tested at the same time, using a radial immunodiffusion (RID) assay to quantify the serum IgG level and ATR spectroscopy to acquire the IR spectrum for each sample.
The diffraction spectrum for each sample was recorded for 28 s.
The average of the replicate spectra for each sample (after removal of outliers) was used for subsequent analysis.
It is found that the O 1 s spectrum for each sample can be well separated into three peaks, which are located at 529.8, 531.0, and 532.0 eV, respectively.
Therefore, the spectrum for this sample predominantly reflects the structure of protein molecules in the aggregates.
The final spectra for each sample were the result of 128 scan averaging at high resolution (4.8 8.9 cm-1) with a 3.1 micron spot size.
MIR microscopic imaging spectra were log 1/ R transformed in the first step and an average spectrum for each sample was recorded.
The spectra recorded for each sample were corrected by subtraction of the signal provided by the buffer in the corresponding cell.
A computer performed a Fourier transform on the measured interferogram to obtain an infrared spectrum for each sample location.
The UV vis absorption spectra of the TiO2 composite nanofibers with different vermiculite mass fraction are compared in Fig. 5a, displaying the absorption spectra for each sample for an excitation wavelength of approximately 400 nm (3.10 eV).
Figure 10a and 10b show the PL intensity at 10 K for samples A and B, respectively, where the emission spectra, for each sample type, collected with two linear polarizations, namely: along [ 0 1 ¯ 1 ] and along [011].
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