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Stress strain curves were obtained for each specimen from the load (F) vs. displacement (ΔL) data acquired, taking into consideration the dimensions of the specimens, area (A) and length (L), with diameter and height measured three times.
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Zero-field imaging was performed in the JEOL 2100F-LM, which has a residual out-of-plane field in the specimen area of <0.4 mT and a spherical aberration coefficient, Cs, of 109 mm.
The thickness of the conducting nano-carbon layer does not need to be explicitly known, but it is assumed that the lateral dimension is uniform over the specimen area.
The ionization of the gas molecules can be caused both by primary electrons entering the specimen area and by secondary electrons ejected from the specimen.
4. Wash the slides in PBS, blot dry except for the specimen area and then place on a level surface in the humidity box. 1. Flood slides with the previously assessed working dilution of HRPO-labelled anti-rinderpest immunoglobulins in PBS. 2. Close the lid of the humidity box and incubate at 37°C for two hours.
First, because all images in a tomographic tilt series are collected from the same specimen area, the cumulative dose has to be restricted to the level used in single-particle work to obtain a single image.
The associated electron dose can be estimated from the irradiated specimen area of 1.0 µm2 (note that the area actually shown in the inset of Figure 6C is smaller), which gives an electron dose of 1⋅104 electrons/nm2.
The fringing electric field increases the apparent relative permittivity of cement when the electrodes do not cover the entire specimen area.
Complementary (S TEM measurements reveal the location of threading dislocations and the presence of antiphase boundaries at the same specimen area.
Jinschek and Helveg additionally speculated that a local charging of the illuminated specimen area could be causing motion of the specimen through a capacitive effect, whereby smearing of the smallest image features leads to degradation of the resolution.
In this approach, the same specimen area is imaged many times at different tilt angles, and the images are computationally combined to generate a density map of the imaged specimen.
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