The third schedule involved rejuvenation of the specimen above Tg between each measurement.
The shape memory behavior was triggered by heating the specimen above the melting point of the crystalline soft segment.
However, single-photon excitation used in CLSM results in broad exposure to high energy UV and visible excitation sources in the specimen above the focus plane, which can reduce the viability of photosensitive, living samples [ 2].
Some of the specimens discussed above have only been recently announced, and most of them are still being worked on by the teams that discovered and described them, making it difficult for other researchers to independently assess them.
Total RNA was extracted from swine clinical specimens (above) as previously described, including a positive extraction control that was prepared to yield a pre-determined Ct value [33].
The manufacturing route adopted for the auxetic PU specimens involved a multiaxial compression of the native foam, heating of the compressed specimens above the Tg of the foam polymer, and cooling under running water.
PL from Er3+ ions could only be clearly observed in the specimens annealed above 900°C, and its intensity increases by a factor of 12 with the annealing temperatures until 1,100°C, followed with a sudden drop at 1,150°C.
The Vironostika negative control, CDC calibrator, CDC high positive control and CDC low positive control were all diluted and incubated in the same manner as the specimens described above.
All the specimens mentioned above were examined with an Axioscop 2 plus (Carl Zeiss, Göttingen, Germany), MZ 16 FA (Leica Microsystems, Wetzlar, Germany), or FV1000 confocal laser microscope (Olympus, Tokyo, Japan).
Note 3 – While the paper in which Yulania cuneatofolia is named as a new species is titled "Two new species of Yulania Spach from Hubei Province", this species is only noted in that paper as occurring in Henan Province, as for the type specimens above.
