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The specificity of this method was validated against a total of 32 strains representing 19 different bacterial species.
The sensitivity and the specificity of this method decrease in adult patients, due to the lower prevalence of gastric mucosa in symptomatic Meckel's diverticulum in adult patients [2].
The peak purity factor was more than 980 for drug product (Table 6), which further confirmed the specificity of this method.
In order to increase the specificity of this method, the sequence length of the stem-loop RT primer to individual miRNAs was extended to 8 nucleotides complementary to the 3' end of the miRNA.
While the specificity of this method approaches 100%, the sensitivity ranges from 55 to 92% [ 68, 69].
The specificity of this method is determined by the primers' specificity and by the melting temperature (Tm) of the amplicon.
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The specificity of this detection method, evaluated by the comparison of the results of Liseria monocytogenes, E. coli O157, and B. melitensis 16M is shown in Fig. 8a.
The specificity of this detection method was evaluated by the comparison of the sensing results of L. monocytogenes (ATCC 19115), E. coli O157 (ATCC 35150), and B. melitensis 16M.
The similarity in peak and shape of the two spectra show there is no overlap band from other components of coffee in these regions, and this shows the specificity of the method.
This increases the specificity of the method by recovering a larger fraction of RBP-bound RNAs and the removal of less specifically bound RNAs through more stringent washing steps during the immunoprecipitation.
This will increase the sensitivity while reduce the specificity of the method.
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