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The mice were allocated to the treatment groups as matched pairs, according to the severity of the first peak.
The mice were allocated randomly to the experimental groups and were acclimatized for 1 2 weeks before the start of experiment.
Therefore, the mice were allocated to one of the three groups: mice without adenovirus injection + ND, mice injected with adenovirus containing GFP (Ad-GFP) + HFD (high-fat diet, 60% kcal from fat, TD.06414) (Harlan Teklad, IN, USA), and mice injected with adenovirus containing JMJD2B (Ad-JMJD2B) + HFD.
The other 60 mice were allocated into 4 treatment groups.
Eight weeks later, the mice were allocated to different treatment groups.
A total of 172 WT mice were allocated into the vehicle group and 216 into the EC groups.
After getting the random number by computer-generated randomization, C57/BL6J male mice were allocated in a blinded fashion.
A total of 75 mice were allocated into 9 groups.
Then 30 mice were allocated into 3 groups of 10 mice per group.
No specific funding was allocated to this study.
The area of EU27 was allocated to the responsibility of on specific plot level model.
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