Sentence examples for the specific inserts from inspiring English sources
The presence of the respective construct in the transformants obtained was confirmed by PCR on DNA extracted from the different colonies using specific oligonucleotides for the specific inserts.
The recombinant clones having the specific insert in the forward orientation was then sequenced from both directions to get a contiguous sequence in an automated sequencer (ABI prism, model 377, Version 3.0).
Accordingly, the MW for the recombinant proteins was calculated as a sum of the specific insert and the MW for the GvpC.
As would be anticipated, these show that the SIVsm specific staining appears to become more diffusely distributed in the cells both as a function of the elapsed time, and the specific insert.
In case of the cellZscope the membrane area of the specific insert type is considered by the software and the TEER is automatically normalized and always given in Ω · cm.
Results from the subsequent immunostain monitoring of both the gas vesicle protein GvpC, and the SIV specific inserts confirmed their slow, progressive disappearance over time.
In the context of a display/delivery particle, it is important that cells can both internalize the r-GVs, and successfully degrade them, allowing release of the pathogen specific insert they carry.
Plasmids with verified sequences were digested with EcoRI to release fragments containing the 1lox and 2lox specific inserts, respectively.
Typical plant AP preproteins contain a C-terminal domain of approximately 50 100 amino acids (called the plant specific insert, PSI) which is removed during protein maturation.
Other functionally important inserts such as the Plant Specific Insert in phytepsins have been identified (Runeberg-Roos et al. 1991) and recently a comparative genomics study of APs in Oomycetes demonstrated that a substantial number of APs have sequence characteristics, such as potential membrane spanning stretches, that suggest different functions (Kay et al. 2011).
Membranes were probed with P-labelled probes made from gene specific inserts represented in the microarray analytical results.
