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the spb
initialism
Shortest Path Bridging (SPB); IEEE standard 802.1aq.
Exact(60)
Because Kar5p localizes near the SPB, and because the SPB-enriching factor is also present in mitotic cells, we suspected that Kar5p is anchored by an SPB half-bridge protein.
We observed that Kar5-TM3-GFP still enriched at the SPB in mitotic cells, demonstrating that the SPB-enriching factor is not restricted to pheromone treated cells.
We accordingly examined whether Pcp1 is involved in Cut12 recruitment to the SPB, but again Cut12 GFP localised normally to the SPB in pcp1-18 cells at 36°C.
The significance of this is unclear, although we note that there is still some Prm3p enrichment at the SPB even in the absence of Kar5p, suggesting that Prm3p recruitment near the SPB involves both Kar5p-dependent and Kar5p-independent interactions.
Indeed, some EMs show the SPB within 50-100 nm of the plasma membrane, and so it is possible that the SPB may directly contact the cortex.
As the MI spindle formation started at around 160 min, our observation suggests that the SPB is in the duplicated status for about 100 min, during which time the rMTOC is expected to decorate the SPB.
The label was confined to an area just outside the SPB and stretched out to a distance of 150 350 nm from the SPB (measured on 20 serial sectioned SPBs).
Since 1997, we have developed the SPB.
The dynamic range of the SPB is ± 100 V.
Reducing the spb parameter will reduce the sweep time, making the output more suitable for resolving short signal events.
When the spb parameter is set to 16,777,216, the sampling time is around 0.67 s.
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