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The relatively low tumorigenicity detected in the sorted population in general is likely due to impaired viability of the cells by the sorting procedure.
RNA integrity of the sorted samples was determined using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), since the effect of the sorting procedure on RNA integrity was not known.
These amoebae are heterotrophs based on the sorting procedure that excluded photosynthetic cells (see Methods) and the absence of plastid DNA in the assemblies.
Finally, a population of an exact size of n pop is obtained using the sorting procedure.
On the other hand, the fixed antenna selection need not the sorting procedure.
The sorting procedure consists of cards with items that describe children's behaviors with mothers in stressful situations.
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For cell culture, all the sorting procedures were carried out under sterile conditions.
Moreover, the sorting procedures allowed a pure collection of host and tumor cells that could be further characterized at the genomic and proteomic level.
Most importantly, the low shear forces of this device ensured that no significant damage occurred to the cells during the sorting procedures, which allowed subsequent applications for other bioassays.
To test the influence of the cell sorting procedure on the activation state of the cells (exemplified on CD14+ cells), 2.5 mL aliquots of whole blood stored in EDTA for 20 minutes were exposed to magnetic beads coated with anti-CD14 antibody or incubated without magnetic beads ("PAXgene 3", "PAXgene 4", experimental group 3 and 4, respectively).
For sorting, the GFP+ and GFP++ NPs populations were collected (the GFP negative cell fraction was discarded) and cell viability at the end of the FACS sorting procedure was determined using trypan blue dye exclusion method.
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