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The drying process of the solution was monitored by dynamic optical microscopy (OM) with a digital video camera.
The change occurring in the solution was monitored visually as well as by UV-vis spectroscopy.
The reduction process of the solution was monitored on a Perkin Elmer double-beam UV spectrophotometer.
The reduction process of the solution was monitored on a PerkinElmer double-beam UV spectrophotometer (PerkinElmer Inc., Waltham, MA, USA).
The bioreduction of silver ion in the solution was monitored using Perkin Elmer Lambda 2 double beam UV-visible spectrometer (Waltham, MA, USA) against distilled water as blank.
The reduction of Au3+ ions in the solution was monitored at periodic intervals with the help of UV vis spectrophotometer (Shimadzu 2450).
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The conductivity of the solution is monitored and the CSD is sampled out as a function of time.
The physicochemical parameters, including pH, redox potential (ORP), the concentration of dissolved ferrous iron, total iron, copper ion, zinc ion and sulfate ion in the solution were monitored and a flask of each experimental group was removed for DNA extraction on day 6, 12, 21 and 30 (Xiao et al. 2015).
The NLP solutions were prepared as before, and after transferring to transparent plastic cuvettes, the optical density of the solutions was monitored by reading the A650 with a spectrophotometer.
The absorption kinetic profiles of the solutions were monitored by measuring absorbance at 578 nm in the time range 20 180 s after initiation of the reaction with 2 s intervals.
The laccase activity and the pH in the solutions were monitored regularly.
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