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The solution was kept at 70 °C during the whole deposition procedure.
Then the solution was kept under a desiccator until crystalline AlCl3.6H2O formed.
The solution was kept aside for crystallization.
The solution was kept for half an hour for stirring.
The solution was kept boiling and stirring for 60 min.
The acidity of the solution was kept at pH = 5.
The solution was kept undisturbed for 24 h.
The temperature of the solution was kept at 10 °C.
After adding the APS, the solution was kept on stirring for 3 h.
The stirring was stopped and the solution was kept undisturbed for 24 h.
For complete precipitation of the IgG, the solution was kept over ice for 3 h.
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