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At the injection site, 0.3 μL of the solution was injected bilaterally at a rate of 0.1 μL/min, and the needle was left in place for 5 min to allow the neurotoxin to sufficiently diffuse away from the needle tip.
After exposure, the contrast medium - a mixture of glycerol (70%%) and barium sulfate (30%%) - was injected in these tissues using a previously described technique (Van Hoof et al. 2013): A thin needle (0.45 mm × 23 mm) was inserted between the collagen fibers of the explored tissue, and the solution was injected with mild pressure until leakage occurred at the injection site.
We found huα-syn-positive cells around the injection site in the overlying cortex when the solution was injected into the LV.
Then, the solution was injected rapidly into the hot reaction solution at 270°C under N2.
A 25-μL aliquot of the solution was injected into the UFLC system for the analysis.
The concentrated residue was dissolved in 100 μL of methanol and a 1.0 μL sample of the solution was injected into the GC-MS system.
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The tendon significantly expands as the solution is injected (2b).
In the spray drying method, when the solution is injected into the chamber through the nozzle, atomization caused by compressed air breaks down the solution into small droplets.
Lignin acetate (0.5 mg) was dissolved in 1 mL of tetrahydrofuran, and 30 μL of the solution were injected.
The solutions were injected in triplicate into the HPLC column, keeping the injection volume constant (20 μL).
The solutions were injected and the signal to noise ratio (S/N) was recorded.
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