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The solution was extracted with DCM (six times 20 mL).
The solution was extracted twice with 5.0 mL of ethyl acetate.
The solution was extracted with chloroform (150 × 2 mL) and washed with water (50 × 2 mL).
After filtration, the needles were dissolved in saturated NaHCO3 and the solution was extracted with DCM (three times 20 mL).
The solution was extracted with diethyl ether (80 × 3 mL) and the organic layer was washed with water (70 mL).
After the absorption equilibrium was reached, the solution was extracted by syringe filter for determination of benzene concentration.
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Photosensitizers prepared by covalent attachment of 1-methylimidazole to aryl ketones are soluble in ionic liquids and remain in the ionic liquid layer when the solution is extracted with an organic solvent.
For the analyses of authentic standards and urine samples, the same extraction conditions with the SPME fiber were used and the solutions were extracted and measured once.
To evaluate the miRNAs remaining in the AMV solution, the rest of the solutions were extracted with Trizol to obtain the RNA contents.
The solutions were extracted twice at acidic pH 2.0 (6N HCl) with ethyl acetate.
After incubation, the solutions were extracted with 2× volume phenol/chloroform and twice with 2× volume chloroform.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com