We have pointed out that in the case of the usual NNK codon degeneracy encoding all 20 canonical amino acids as building blocks, protein sequence space and the respective oversampling increase astronomically as the size of a randomization site increases from one residue to larger ones, e.g., 10.
The envelopes were only accessed when both personnel were present, and the size of the randomization blocks was not known.
We further tested this hypothesis in a mouse xenograft model by focusing on the tumor response defined by tumor regression to half of the size of the tumors at randomization.
Significance of topological congruence was inferred by a randomization test concerning the size of the MAST.
Both spider and skylark were negatively affected by patch size randomization, which reduced the sizes of the agricultural fields in which these species prosper.
Other relevant limitations of this study are the lack of a control group, the relative small sample size, the lack of randomization and the absence of a follow-up.
Despite the large size of the study, randomization failed to balance important baseline patient characteristics, such as severity of illness, presence of mechanical ventilation, and post-operative status.
Although this study showed impressive recovery in visual acuity that is about 5-fold faster than that expected after occlusion therapy, the authors also point out that the study contains several limitations: small sample size, lack of randomization, and differences in number of groups.
The following quality items were used to assess the methodological quality and risk of bias in the studies [[30],[31]]: eligibility criteria, adequacy of sample size, reporting of randomization, reporting of blinding, avoiding selective reporting, description of intervention details, description of outcome measures, description of adverse effects, and adequacy of data analysis.
The limitations of this study include its retrospective nature, small sample size, absence of randomization, and short follow-up period.
