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At the single transcript level, there was no biological evidence of altered gene expression in PBLs that correlated with chronic, impairing, and medically unexplained fatiguing illness.
Following the single transcript and gene set level analyses, we assessed the dynamic transcriptome from a systems-level point of view.
samples, while the single transcript more highly expressed in spleen i.n. was IGKV1-88_AJ231206_Ig_kappa_variable_1-88_289, possIgGy an IgG kappa chain.
We have discovered a higher percentage of ToxoDB gene model inaccuracies, especially in UTR and terminal exon regions, than previously reported [ 7] and report on alternative transcript isoforms as opposed to the single transcript per gene currently reported in Toxodb.
Apparently for the single transcript gene the LRTS insertion either has not disrupted the host gene function or possibly provided some beneficial modulation of the initial function and thus has been tolerated by natural selection.
In order to analyze the transcriptome on the single transcript level, all sequences for each species were assembled into a gene index resulting in a total of 116,207 unique sequences over all six species.
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Many of the single transcript-tags came from MD 80228, which had the highest bacterial diversity profile of all libraries analyzed, and from MD 84730.
Some of these events can now be observed and measured at the single-transcript level.
The single-transcript organization was confirmed by RT-PCR using a pair of primers encompassing snoR775 and miR775 (data not shown).
Alternative splicing in the primary transcript results in multiple FN transcripts of the single copy gene.
In that study, a bicistronic reporter system was used that included KalTA4 (an optimized version of the Gal4-activator) downstream of a 4×UAS-cassette with EGFP and a T2A sequence, allowing expression of two proteins from the same single transcript (Szymczak et al., 2004).
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