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The short reads generated in the sequencing of each DNA molecule can be counted and quantified, allowing the identification of mutations in nonmodal populations of cells (that is, identification of a somatic mutation in a small subpopulation of cells immersed in a modal population with wild-type sequences) and accurate copy number assessment of each genomic region ([ 14] and references therein).
The sequencing of each gene showed the chimeric CYP21A1P/CYP21A2 gene next to the TNXA pseudogene, and the presence of a wild-type CYP21A2 next to the TNXB gene.
Primers used for the sequencing of each gene are listed in the Table.
Further details regarding the sequencing of each library is provided in Additional file 1: Table S6.
Additionally, nested (internal) primers were also designed to complete the sequencing of each fragment (Additional file 4).
It allowed for the sequencing of each allele separately, and therefore, for the detection of haplotypes (i.e. which codon 72 variant co-localized with which PIN3 variant).
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After the sequence of each fragment is determined, powerful computers assemble the pieces in the correct order by looking at overlapping sequences.
Using a technique called mass spectroscopy, it is determining the sequence of each protein's amino acids, the building blocks of proteins.
This barrier is strongly influenced by the charges partitioned along the sequence of each channel.
The sequences of each gene or region were aligned by MUSCLE with default parameters.
The sequence of each antisense DNA oligonucleotide DNA and the respective abbreviations are listed in Table 1.
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